ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the protective effect of S-isopentenyl-L-cysteine,a new cysteine derivative,on DNA damage induced by radiation by using acute radiation injury animal models.</p><p><b>METHODS</b>Forty ICR mice were randomly divided into five groups:the control group,1.0Gy gamma irradiation group,1.0Gy gamma irradiation combined with S-isopentenyl-L-cysteine group,7.2Gy gamma irradiation group,and 7.2Gy gamma irradiation combined with S-isopentenyl-L-cysteine group,with 8 mice in each group.The comet assay and bone marrow polychromatic micronucleus experiments were performed to evaluate the double-strand DNA breaks in ICR mice exposed to 1.0 and 7.2Gy gamma-ray, respectively.</p><p><b>RESULTS</b>The tail DNA percentage,tail length,tail moment,and olive tail moment of peripheral blood lymphocytes in 7.2Gy gamma irradiation group were significantly higher than that of the control group (P<0.01).And it was also observed that above experimental indexes of 7.2Gy gamma irradiation combined with S-isopentenyl-L-cysteine group was significantly less than that of 7.2Gy gamma irradiation group (P<0.05). In addition,the micronucleus rate of 1.0Gy gamma irradiation group and 7.2Gy gamma irradiation group were both significantly higher than in the control group (P<0.01). In addition,in mice given S-isopentenyl-L-cysteine before irradiation,the micronucleus rate of ICR mice exposed to 1.0 and 7.2Gy gamma-ray decreased from (39.5000 ± 3.3141)‰ to (28.1667±4.1345)‰ (P=0.033) and from (76.5000 ± 4.6242)‰ to (22.8333 ± 3.6553)‰(P=0.000),respectively. The bone marrow polychromatic micronucleus experiment indicated that the value of polychromatic erythrocyte (PCE)/normochromatic erythrocyte(NCE) of ICR mice exposed to 1.0 and 7.2Gy gamma-ray was less than the control group(P<0.05). Meanwhile,after irradiating by certain dose,the value of PCE/NCE in mice given S-isopentenyl-L-cysteine before irradiation was significantly higher than the corresponding groups (P<0.05).</p><p><b>CONCLUSION</b>S-isopentenyl-L-cysteine has a good protective effect against DNA damage induced by radiation.</p>
Subject(s)
Animals , Mice , Bone Marrow , Cysteine , DNA Damage , Disease Models, Animal , Gamma Rays , Mice, Inbred ICR , Radiation Injuries , Radiation-Protective AgentsABSTRACT
<p><b>OBJECTIVE</b>To observe the in vivo effects of oxysophoridine on hepatocellular carcinoma in mice and to study the related mechanisms.</p><p><b>METHODS</b>C57BL mice were inoculated with mouse hepatoma H22 cells subcutaneously, then divided into 5 groups (14 per group), and treated with oxysophoridine (50, 100, or 150 mg/kg) or cisplatin (4 mg/kg) for 10 days. Inhibitory rate of tumor, body weight gain, and influence indices on internal organs (liver, spleen and thymus) were evaluated. The differentially expressed genes between the oxysophoridine-treated group, and the control group were analyzed using cDNA microarray and quantitative real-time PCR (qRT-PCR) experiments.</p><p><b>RESULTS</b>Compared with the tumor weight of the control group (2.75±0.66 g), oxysophoridine significantly suppressed hepatocellular carcinoma growth in mice (P <0.01), with 0.82±0.36 g, 0.57±0.22 g, and 1.22±0.67 g for the tumor weight in the low, moderate, and high dose treatment group, respectively. The moderate dose led to the highest inhibitory rate, 79.3%. Observation of body weight gain and influence on three organs showed that compared with cisplatin, oxysophoridine produced fewer side effects in vivo. cDNA microarray and qRT-PCR showed that the most significant differentially expressed genes in the tumor samples of oxysophoridine-treated mice were mostly involved in regulating apoptosis, with the Tnfrsf11b (osteoprotegerin) gene being the most significantly affected.</p><p><b>CONCLUSION</b>Oxysophoridine was a promising compound for developing drugs against hepatocellular carcinoma, and its anti-hepatoma effect was probably related to osteoprotegerin activation.</p>
Subject(s)
Animals , Mice , Alkaloids , Pharmacology , Carcinoma, Hepatocellular , Genetics , Pathology , Cell Line, Tumor , Cell Proliferation , Drug Screening Assays, Antitumor , Gene Expression Regulation, Neoplastic , Liver Neoplasms, Experimental , Genetics , Pathology , Mice, Inbred C57BL , Oligonucleotide Array Sequence Analysis , Organ Specificity , Real-Time Polymerase Chain Reaction , Tumor Burden , Weight GainABSTRACT
<p><b>OBJECTIVE</b>To investigate the anticancer activity of the total flavonoids isolated from a herbal formula, Xianhe Yanling Recipe (仙鹤延龄方), a recipe commonly used in cancer patients in China.</p><p><b>METHODS</b>The in vitro anticancer activity of the total flavonoids was determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on three cancer cell lines: MCF-7 (a human breast adenocarcinoma cell line), HepG-2 (a human hepatocellular carcinoma cell line) and ES-2 (a human ovarian cancer cell line). The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma, and cisplatin was used as a positive control.</p><p><b>RESULTS</b>The total flavonoids exerted a powerful inhibitory effect on the three cell lines, with 50% inhibiting concentrations (IC(50)) of 24.948, 31.569 and 6.923 μg/mL, respectively. In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.</p><p><b>CONCLUSION</b>The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity, and further researches and development are warranted.</p>
Subject(s)
Animals , Humans , Mice , Antineoplastic Agents , Pharmacology , Cell Line, Tumor , Cell Proliferation , Drug Screening Assays, Antitumor , Drugs, Chinese Herbal , Pharmacology , Flavonoids , Pharmacology , Inhibitory Concentration 50 , Neoplasm TransplantationABSTRACT
Theory of traditional Chinese medicine (TCM) properties, the essence of TCM theories, is the treasure for the Chinese nation, even for the world. It's an emergency to illustrate this theory in modernizing meaning so as to share it, one of the achievements in the Chinese civilization, for all the people in this world. TCM property-matteromics, the new concept in this article, is reported at the first time. Defined as the science of studying composition of the basic materials expressing TCM properties, correlation and interact between these materials, TCM property-matteromics was presented here in expounding the basic concept, the object, content and method of study, in order to apply a new thinking and new methodology for the TCM modernization by elucidating the essence of the theory of the TCM property.
Subject(s)
Drugs, Chinese Herbal , Medicine, Chinese Traditional , MethodsABSTRACT
In this article, the literatures concerning the mechanism of TCM in anti-tumor and tumor metastasis prevention in recent years were reviewed and summarized into categories of effective components, effective portion, extraction of single drug, and TCM compound.
Subject(s)
Humans , Adjuvants, Immunologic , Pharmacology , Therapeutic Uses , Angiogenesis Inhibitors , Therapeutic Uses , Antineoplastic Agents, Phytogenic , Therapeutic Uses , Drugs, Chinese Herbal , Therapeutic Uses , Neoplasm Metastasis , Neoplasms , Pathology , Phytotherapy , MethodsABSTRACT
With the imformation technology getting a great progress in recent years, the modem imformation technology is extensively employed in the study of traditional Chinese medicine (TCM) prescriptions. In this article a summary is given, which includes applications of modern imformation technology in the study of TCM presciptions. It focus on the introduction of the databse technology, data-mining technology and chemometrics, and brief virtual screening technology, experimental design, innovation design, study of complexity and bioinformatics technology, all of which deployed in the study of TCM presciptions, so as to enligten researchers on modernized study of TCM prescription and its development in the future.
Subject(s)
Humans , Databases as Topic , Drug Combinations , Drug Information Services , Drugs, Chinese Herbal , Chemistry , Medicine, Chinese Traditional , Methods , Plants, Medicinal , Chemistry , Prescriptions , Quantitative Structure-Activity Relationship , SoftwareABSTRACT
The main pharmacological constituents of Chinese traditional medicine herb Cnidium monnieri are coumarin compounds and volatile oil. In addition, it contains monoterpene polyols, glucides, as well as recently discovered sesquiterpene components. In recent years, rather active investigations of its anti-tumor were performed at home and abroad. C. monnieri possesses multi-aspect and comprehensive anti-tumor functions, involving directly tumor-inhibitory activity, anti-mutagenicity, reversing multi-drug tolerance of tumor, as well as improving immune functions and so on. In this review, chemical constituents, anti-tumor activities and relevant investigations of Fructus Cnidii were summarized recent decade.
Subject(s)
Antineoplastic Agents, Phytogenic , Pharmacology , Cnidium , Chemistry , Coumarins , Chemistry , Pharmacology , Drugs, Chinese Herbal , Pharmacology , Furocoumarins , Chemistry , Pharmacology , Molecular StructureABSTRACT
This article reviewed the updated methods and sophisticated technics used in research of traditional Chinese medicinal compound recipes, including mainly biochip technic, computer technic, metabonomics, ADME/Tox technic, fuzzy mathematics, molecular imprinting technic, biotransformation and metabolization in intestinal bacterium and so on.